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May 1963

Isolation of an Alpha Crystallin Antigen from the Chick Iris

Author Affiliations

Departments of Anatomy, and Physiology and Pharmacology, Wayne State University College of Medicine.

Arch Ophthalmol. 1963;69(5):618-621. doi:10.1001/archopht.1963.00960040624015

Antigens that are identical immunologically to chick lens proteins α-, β-, and γ-crystallins have been demonstrated in the chick iris.1,2 The latter tissue can form a lens upon removal of the original lens during embryonic life,3 and it has been suggested that lens antigens in the iris form the substrate for lens regeneration.1,2 Since α-crystallin appears to be the fundamental component in the early development of the lens, it seemed of interest to determine whether the physical structure of the corresponding iris antigen is similar to that of the lens protein.

The present investigation was therefore undertaken in order to isolate and determine the electrophoretic and molecular size properties of the α-crystallin antigen in the chick iris.

Materials and Methods 

Antigens.  —Lenses from adult chick were carefully dissected free from the capsule and surrounding tissue and homogenized in 0.9% saline. After centrifugation for ten minutes at 3,000

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