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November 1963

Further Studies on Binding of Exophthalmos-Producing Substance by Normal Human Serum

Arch Ophthalmol. 1963;70(5):654-658. doi:10.1001/archopht.1963.00960050656014

These experiments were undertaken to study the exophthalmos-inhibiting factor in normal human serum. This factor, described in 1960,1 appeared to be less active in the sera of patients with acute, severe, ocular changes of Graves' disease. Such a finding was of interest, for it helped to explain the apparent lack of correlation between the activity of the thyroid gland and the activity of the orbital process.

Method and Materials  Bioassays were performed on Fundulus heteroclitus, (ie, the killifish or Atlantic minnow) obtained during the summer months from the waters around New York. Measurements of intercorneal distance were made with a micrometer to 0.1 mm. The fish were placed in water in a laboratory finger bowl and readings were taken with the gauge held above the level of the water. Previous studies2 have shown that such measurements are reproducible to within 2.8%, and, therefore, any change greater than 5%