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July 1964

The Fluorescent Antibody Technique in Diagnosis of Herpes Simplex Keratitis

Author Affiliations

Los Angeles
Special Fellow, National Institute of Neurological Diseases and Blindness; present address: Department of (Ophthalmology) Surgery, University of California at Los Angeles Medical Center, Los Angeles, Calif 90024 (Dr. Pettit).; From the Francis I. Proctor Foundation for Research in Ophthalmology and the Department of Ophthalmology, University of California, San Francisco Medical Center.

Arch Ophthalmol. 1964;72(1):86-98. doi:10.1001/archopht.1964.00970020088020

The use of antibody labeled with fluorescein to localize specific antigens in various tissue preparations was originally described by Coons.1 It has since been used as a laboratory means of rapid diagnosis in a variety of microbiological systems and for the study of disease pathogenesis in a number of conditions in which an antigen-antibody system is involved.2

In 1959 Biegeleisen3 used this technique in differentiating clinically similar skin and mucus lesions due to herpes simplex virus and nonherpetic agents. He demonstrated an excellent correlation between the presence of positive fluoresecent staining and the ability to isolate the herpes virus from the lesions.3 The clinical usefulness of this test as a rapid diagnostic tool in herpetic keratitis has been suggested by the preliminary studies of Kaufman4 and Vozza and Balducci.5

The direct technique of Coons is based on the specificity of the antigen-antibody reaction which

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