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December 1964

Histochemical Changes in Endothelium of Corneas Stored in Moist Chambers

Author Affiliations

New York
Fulbright Fellow from the Medical School of the University of Granada, Spain (Dr. Peña-Carrillo).; From the Department of Ophthalmology and The Corneal Center, Columbia-Presbyterian Medical Center.

Arch Ophthalmol. 1964;72(6):811-816. doi:10.1001/archopht.1964.00970020813015

Introduction  Experimental and clinical studies have shown that a normal, functioning endothelium is necessary in order to obtain successful penetrating keratoplasties.1,2 This is in contrast to the good results obtained with preserved corneas in lamellar grafts1-3 where the absence of corneal cells does not interfere with its transparency. It has been shown by Schaeffer,4 in electron microscope studies, that swelling of mitochondria of endothelial cells of corneas preserved in moist chambers at 4 C appears at 24 hours of storage, and that irreversible structural changes occur only after 96 hours of storage. Electron microscope studies, as well as culture of cells obtained from stored or preserved corneas, made with the purpose of determining the viability of the tissue, in a sense, do not show a true picture of what is happening in the corneal endothelium as a whole because they cannot give a panoramic view of the