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March 1965

Inhibition of a-Chymotrypsin by Aqueous Humor

Author Affiliations

From the Department of Ophthalmology, Hospital of the University of Pennsylvania, Philadelphia General Hospital, Veterans Administration Hospital, the Children's Hospital of Philadelphia, and from the Laboratory of Surgical Pathology and the Harrison Department of Surgical Research, Hospital of the University of Pennsylvania.

Arch Ophthalmol. 1965;73(3):399-401. doi:10.1001/archopht.1965.00970030401019

The inhibitory effect of aqueous humor on α-chymotrypsin has recently been demonstrated by Bedrossian and Weimar.1 In the discussion of this paper, however, Dr. B. Schwartz raised the objection that the colorimetric technique, which the authors used, only demonstrated inhibition in terms of liberation of tyrosine groups from hemoglobin molecules. It seemed worthwhile, therefore, to test the effect of aqueous humor on α-chymotrypsin by using a different substrate and a different method.

Material and Methods  On four consecutive operating days the aqueous humor was collected from the anterior chamber on all routine, uncomplicated cataract sections. A conjunctival flap was reflected and dissected into the cornea for about 1 mm. A knife needle was inserted into the anterior chamber and followed by a 27 gauge blunt needle through which aqueous was aspirated. Fluid was collected from a total of 14 eyes and pooled each day. Aqueous was obtained from four

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