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March 1965

Mechanisms of the Host Response in the Eye: I. Changes in the Anterior Eye Following Immunization to a Heterologous Antigen.

Author Affiliations

San Francisco
From the eye research laboratories, Department of Ophthalmology, University of California School of Medicine, San Francisco General Hospital; the clinical laboratories, San Francisco General Hospital; and the Francis I. Proctor Foundation for Research in Ophthalmology.

Arch Ophthalmol. 1965;73(3):402-412. doi:10.1001/archopht.1965.00970030404020

Introduction  Descriptive morphologic classification of lesions of the anterior eye represents the major classifying system in clinical ophthalmology. Epidemiologic studies have correlated many of these pictures with the presence of infectious agents, noxious stimuli, or presumed allergenic agents.1 In some cases histopathologic data has also been accumulated.The studies to be presented were initiated with the hypothesis that many of the described morphologic changes represent successive immunologic stages which can be demonstrated in the rabbit eye by conjunctival application of a nonreplicating heterologous antigen.

Materials and Methods 

Antigen Solution.  —BSA (bovine serum albumin, Nutritional Biochemical Company) was used as one immunizing antigen. For conjunctival immunization the antigen was mixed in sodium phosphate buffer (0.1 M, pH 6.8) at a concentration of 150 mg/ml. For systemic immunization the antigen was mixed with complete Freund's adjuvant, 1:1, or prepared as an alum precipitate (100 mg BSA/5.0 ml of 1% aluminum ammonium

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