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March 1965

Fluorescent Antibody Localization of Herpes Simplex Virus in the Conjunctiva

Author Affiliations

San Francisco
From the Francis I. Proctor Foundation for Research in Ophthalmology and the Department of Ophthalmology of the University of California, San Francisco Medical Center. This investigation was conducted under a Fight for Sight Research Fellow of the National Council to Combat Blindness, Inc., New York, and was supported in part by the United States Public Health Service Research Grant No. NB 02542.; Fight for Sight Research Fellow of the National Council to Combat Blindness, Inc., New York, 1963-1964, Department of Ophthalmology, Tokushima University, School of Medicine, Tokushima, Japan (on leave) (Dr. Uchida).

Arch Ophthalmol. 1965;73(3):413-419. doi:10.1001/archopht.1965.00970030415021

Introduction  Experimental herpes simplex infection of the rabbit eye resembles primary ocular herpes in man. As in man, conjunctivitis is often associated with the keratitis and may be more severe than the keratitis. Evidence for the presence of virus infection in the cornea has been well documented over the years. Scrapings from the dendritic lesions show the viral type of giant cells. Intranuclear inclusions are not usually visible in epithelial scrapings but can be seen if the cornea is sectioned. Specific localization of the viral antigen can be regularly demonstrated by staining the epithelial scrapings with specific fluorescein-tagged antisera (FA stain).1-3 Recently Pettit et al4 correlated this localization of the antigen by FA staining with its localization by the electron microscope.Although the conjunctivas of eyes inoculated with herpes virus become inflamed, definite conjunctival lesions have not been clinically demonstrable. Experimentally, however, a few observers—notably Goodpasture and Teague

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