Meridional sectioning of the cornea allows only very limited examination of the endothelium; therefore, other methods have been devised which allow larger portions or even the entire endothelium to be studied in one preparation.1-3 However, these alternate methods can be very time consuming and require a considerable degree of dexterity. Further, some of these methods have not been applied successfully to human corneal endothelia.When applied to edematous corneae, those techniques which require separation of Descemet's membrane and of endothelium from the remaining cornea may cause some loss of endothelial cells from Descemet's membrane. This loss is obvious in a comparison of the endothelium of an edematous cornea before and after separation with Descemet's from the underlying stroma.
The method described below provides a permanent preparation of complete endothelium with adequate visualization of cellular details, and has the further advantages of simplicity, decrease in possibility of cell
NICHOLAS JP. Whole Mounts of the Corneal Endothelium. Arch Ophthalmol. 1965;73(4):555–558. doi:10.1001/archopht.1965.00970030557020
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