[Skip to Content]
[Skip to Content Landing]
June 1965

Abolition of the Electroretinogram: Following Injection of α-Chymotrypsin Into the Vitreous and Anterior Chamber of Monkey

Author Affiliations

Miami, Fla
From the Bascom Palmer Eye Institute, University of Miami School of Medicine, Department of Ophthalmology.

Arch Ophthalmol. 1965;73(6):843-850. doi:10.1001/archopht.1965.00970030845017

Introduction  The infusion of α-chymotrypsin into the anterior chamber of the eye during cataract surgery has raised important questions on the effect of this proteolytic enzyme on various eye structures. The studies to date have used histological methods to assess the changes and have shown, not surprisingly, that the enzyme can damage tissues of the eye. Thus Maumanee (1960) reported that injection of 15 units (see Schwartz and Schwartz, 1960) of α-chymotrypsin into the vitreous of rabbits produced localized atrophy of the retina with the supporting structures of the retina most affected.Radnót and Pajor (1959) similarly noted damage to the retina of the rabbit although they did not state the dosage or route of administration of the enzyme. In the dog, O'Malley et al (1961) reported that injection of 0.4 cc of 1/5,000 parts of α-chymotrypsin (about 60 units) produced a high incidence of retinal damage. The rods and