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January 1966

Ganglion Cells in the Human Ciliary Body

Author Affiliations

From the departments of ophthalmology and pathology of the University of Michigan Medical Center.

Arch Ophthalmol. 1966;75(1):57-60. doi:10.1001/archopht.1966.00970050059011

Ganglion cells have been identified in the human ciliary body by histochemical demonstration of the cholinesterase in their cytoplasm.

Routine histological preparations as well as silver stains reveal occasional bipolar or multicentric ganglion cells in the choroid of the normal human eye.1-4 No ganglion cells have been found in the normal iris. Occasional ganglion cells have been recognized in the normal human ciliary body with routine stains as well as with silver techniques.1,5 Technical difficulties, however, have prevented a complete histological identification of these cells in the ciliary body.

Method  The following modification of the histochemical staining technique for cholinesterase by Gerebtzoff6 was used in this study: human eyes were fixed immediately after removal in neutral Formalin at 10 C for 5 to 24 hours. Frozen sections were cut at a thickness of 20μ and placed into cold distilled water. The water was changed three times over