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February 1966

Entrance of Fluorescein Into Aqueous Humor of Cat Eye

Author Affiliations

Leiden, Holland; Bethesda, Md
From the Ophthalmology Branch, National Institute of Neurological Diseases and Blindness, Bethesda.

Arch Ophthalmol. 1966;75(2):247-253. doi:10.1001/archopht.1966.00970050249019

The intravenous administration of fluorescein has been used as an indicator of the permeability of the blood aqueous barrier, and to demonstrate changes of the barrier as induced by pharmacological agents.1,2 It has been reported for the living human eye that certain drugs (pilocarpine, eserine, histamine) facilitate and others (epinephrine, hydrochloride [Adrenalin], calcium, antihistamines) inhibit the entrance of fluorescein.3 There is some doubt whether the entrance of fluorescein is solely from the ciliary processes or whether it emerges also from the posterior surface of the iris. It is also unclear whether fluorescein enters the aqueous by a process of diffusion, filtration, or secretion. For these reasons, experiments were designed on the cat to visualize the entrance of fluorescein in in vivo and in vitro preparations. The in vitro arterially perfused eye was better suited technically for the testing of various physical parameters and to determine the effect of