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June 1966

Amphotericin B in Ocular Histoplasmosis of Rabbits

Author Affiliations

From the Clinical Laboratories of the Jewish Hospital and the Laboratory of Mucology (departments of pathology and dermatology), College of Medicine, University of Cincinnati.

Arch Ophthalmol. 1966;75(6):818-825. doi:10.1001/archopht.1966.00970050820022

Histoplasma capsulatum has never been cultured from lesions of the human eye, even if clinical findings have implicated this organism in the etiology of uveitis.1-10

Amphotericin B (Fungizone) therapy has been highly effective in the treatment of experimental histoplasmosis and in the management of histoplasmosis in man.6 The penetration of amphotericin B into the eyes following intravenous administration has been demonstrated in rabbits.11

Material and Methods  The right eyes of 24 white New Zealand male rabbits weighing initially 1 kg (2.2 lb), were injected with 0.01 ml of saline suspension (1.02× 106/ml) of a 48-hour-old yeast phase culture of H capsulatum (S-76) grown on blood agar at 37 C (98.6 F). Inoculations into the anterior chamber were made after 1 to 2 drops of 0.5% tetracaine hydrochloride were instilled in each eye. The left (control) eye was injected with 0.01 ml of isotonic saline solution, using Hamilton

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