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August 1966

Mucopolysaccharide Staining in Experimental Corneal Edema

Author Affiliations

Los Angeles
From the Division of Ophthalmology of the University of Southern California School of Medicine, and the Estelle Doheny Eye Foundation, Los Angeles.

Arch Ophthalmol. 1966;76(2):238-243. doi:10.1001/archopht.1966.03850010240016

Ashton1 has shown that the staining characteristics of the rabbit corneal mucopolysaccharides are altered by the swelling which follows suspension of the excised cornea in distilled water. A well-standardized corneal lesion resulting in corneal edema can be produced in the live rabbit by instilling into the anterior chamber agents which are toxic to the endothelium. Such an experimental preparation has greater similarities to clinically encountered corneal edema and offers obvious advantages in investigative maneuverability over an in vitro prepared specimen. This investigation demonstrates some of the mucopolysaccharide staining properties of such in vivo edematous corneas produced by the anterior chamber injection of one endothelial toxic substance, Janus green B. Additionally, some observations are recorded on the staining reaction of the normal and the edematous cornea after the subconjunctival administration of methylprednisolone acetate (Depo-Medrol).

Materials and Methods  Albino rabbits weighing approximately 2 kg (4.4 lb) were used. Preliminary experiments to

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