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April 1967

The Fluorescent Antibody Tissue Stain in Experimental Ocular Syphilis

Author Affiliations

Miami, Fla
From the Department of Ophthalmology, University of Miami School of Medicine, Miami.

Arch Ophthalmol. 1967;77(4):530-535. doi:10.1001/archopht.1967.00980020532017

The fluorescent antibody tissue stain for treponemes is described and its usefulness in experimental ocular syphilis documented. Two primates, inoculated by intraocular and intracisternal routes, respectively, acquired syphilis serologically but only one developed clinical lesions. The fluorescent antibody tissue stain (FA) demonstrated the presence of treponemes in aqueous humor from each animal. It was of interest that Treponema pallidum could be demonstrated in the aqueous humor of eyes that were virtually normal at the time of paracentesis. Further investigation of the FA stain in the diagnosis of syphilis is suggested.