Herpes simplex virus antigen was localized in the corneal stroma of rabbits with experimental keratitis by the fluorescent antibody (FA) technique of Coons. Stromal localization appeared to be in keratocytes. The specificity of this localization by the FA stain was proved by electron micrographs.
Naohiko Tanaka, Samuel J. Kimura. Localization of Herpes Simplex Antigen and VirusIn the Corneal Stroma of Experimental Herpetic Keratitis. Arch Ophthalmol. 1967;78(1):68–73. doi:10.1001/archopht.1967.00980030070013