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August 1967

Bacteriologic Study of "Donor" Eyes: Evaluation of Antibacterial Treatments Prior to Corneal Grafting

Author Affiliations

New York
From the Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, Corneal Center (Dr. Polack), and the Department of Microbiology of the Institute of Ophthalmology, Columbia-Presbyterian Medical Center (Dr. Locatcher-Khorazo and Mrs. Gutierrez), New York.

Arch Ophthalmol. 1967;78(2):219-225. doi:10.1001/archopht.1967.00980030221018

A bacteriologic study of 240 eyes obtained postmortem revealed 100% positive cultures. The bacterial flora of these eyes was qualitatively similar to the bacterial flora of normal eyes but there was an increase in the number of gramnegative potential pathogens. Evaluation of two commonly used methods of soaking the "donor" eyes in antiseptic solutions indicated that they did not sterilize the eye but could spread the contamination. It was found that a neomycinpolymyxin B solution used to irrigate the cornea produced negative cultures or notably diminished the contamination of this tissue. Removal of the corneal epithelium with a gauze also decreased the contamination or produced negative cultures in some instances.

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