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December 1967

Endothelial Cell Survival After Keratoplasty in Rabbits: The Effect of Storage of Donor Corneas

Author Affiliations

Bethesda, Md
From the Ophthalmology Branch, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Public Health Service, US Department of Health, Education and Welfare, Bethesda, MD. Dr. Salceda is a PHS International Postdoctoral Fellow from the Department of Ophthalmology and Eye Research Institute, The University of the Philippines, Manila.

Arch Ophthalmol. 1967;78(6):745-752. doi:10.1001/archopht.1967.00980030747011

Transparent full-thickness corneal homografts were obtained in adult rabbits using donor materials that had been stored in +4 C moist chambers for as long as 21 days. At the time of transplantation, the endothelium of the stored corneas presented varying degrees of structural alterations that were related to the duration of storage. When examined at different intervals after keratoplasty, the grafts showed survival of the donor endothelial cells. Cell survival was greater in fresh grafts than in those that had been stored prior to transplantation. The present data indicate that in rabbits a reduction in the population density of the donor endothelium to 50% of the original is still sufficient for maintenance of the homografts in a transparent condition.

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