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January 1969

Isolation of Virus From Herpetic Keratitis: Influence of Idoxuridine on Isolation Rates

Author Affiliations

San Francisco
From the Department of Microbiology and Proctor Foundation for Research in Ophthalmology, University of California, San Francisco Medical Center, San Francisco.

Arch Ophthalmol. 1969;81(1):22-24. doi:10.1001/archopht.1969.00990010024004

Herpes simplex virus can be isolated from active epithelial lesions of herpetic keratitis in 60% to 70% of patients, if a single specimen is inoculated into cultures of an established cell line. However, if the patient has received idoxuridine during the 48 hours preceding the specimen, the chance for isolating virus is drastically curtailed. Only 8.6% of such individuals yielded a virus isolate in spite of the presence of active epithelial lesions. Virus isolation can therefore not be employed as a method to confirm the herpetic etiology of epithelial keratitis which continues to exhibit activity during the administration of idoxuridine.

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