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April 1969

Studies in Uveal Physiology: I. Adaptation of Isotope Clearance Procedures for External Monitoring of Anterior Uveal Bloodflow and Aqueous Humor Turnover in the Dog

Author Affiliations

Washington, DC
From the Division of Ophthalmology, Georgetown University Medical Center, Washington, DC (Dr. O'Rourke), and the Pharmacology Section, Ophthalmology Branch, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Md (Dr. Macri).

Arch Ophthalmol. 1969;81(4):526-533. doi:10.1001/archopht.1969.00990010528011

Turnover measurements were made in canine eyes following direct microinjection and mixture of 1μl to 2μl amounts of serum albumin, iodohippurate, and iodoantipyrene into the anterior chamber (each labeled with iodine 125). Complexity of the procedure was considerably reduced by use of an anterior chamber control cannula for injections and a small scintillation probe for external monitoring. The normal turnover of serum albumin (1.85%/min) was inhibited 75.29% by acetazolamide. lodohippurate and iodoantipyrene cleared at rates of 2.99%/min and 3.85%/min respectively, and each was less inhibited by acetazolamide than was serum albumin. Both hyperoxia and serum uveitis caused significant inhibition of iodohippurate and iodoantipyrene clearances. Significance of the method for studies in uveal physiology is discussed.