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April 1970

Enhancement of the Infectivity Titer of Adenovirus Type 8

Author Affiliations

Iowa City
From the Laboratory of Experimental Ophthalmic Microbiology and Immunology, University Hospitals, the University of Iowa, Iowa City.

Arch Ophthalmol. 1970;83(4):455-457. doi:10.1001/archopht.1970.00990030455013

A successful attempt was made to increase the infectivity titer of the prototype (TRIM) strain of adenovirus type 8, an etiological agent for epidemic keratoconjunctivitis. By use of a washing technique, it was possible to raise this titer by four log arithmic dilutions. Differential ultracentrifugation indicates that the infected particles are brought down at 30,000 rpm with an inhibitor remaining in a supernatant fluid. This inhibitor is somewhat sensitive to the action of α-chymotrypsin, but not trifluorotrichloroethane (Genetron). Coinfection of adenovirus type 8 and Mycoplasma results in adenovirus type cytopathic effects up to a 10−9 dilution.

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