A successful attempt was made to increase the infectivity titer of the prototype (TRIM) strain of adenovirus type 8, an etiological agent for epidemic keratoconjunctivitis. By use of a washing technique, it was possible to raise this titer by four log arithmic dilutions. Differential ultracentrifugation indicates that the infected particles are brought down at 30,000 rpm with an inhibitor remaining in a supernatant fluid. This inhibitor is somewhat sensitive to the action of α-chymotrypsin, but not trifluorotrichloroethane (Genetron). Coinfection of adenovirus type 8 and Mycoplasma results in adenovirus type cytopathic effects up to a 10−9 dilution.
Golden B, McKee AP. Enhancement of the Infectivity Titer of Adenovirus Type 8. Arch Ophthalmol. 1970;83(4):455–457. doi:10.1001/archopht.1970.00990030455013
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