[Skip to Content]
[Skip to Content Landing]
May 1971

Cytologic Study of Herpetic KeratitisPreparation of Corneal Scrapings

Author Affiliations

San Francisco
From the Francis I. Proctor Foundation for Research in Ophthalmology (Dr. Plotkin and Mr. Okumoto), and the Department of Pathology, University of California (Mr. Reynaud), San Francisco. Dr. Plotkin is now with Mt. Sinai Hospital, Cleveland.

Arch Ophthalmol. 1971;85(5):597-599. doi:10.1001/archopht.1971.00990050599013

Intranuclear inclusions were demonstrated in corneal scrapings from rabbits infected experimentally with herpes simplex virus. The most satisfactory method of preparing the scrapings was to fix them with Bouin's solution for one hour, store them in 70% ethanol, and stain them by the Papanicolaou technique. Cytologic study of material prepared in this way could be a helpful diagnostic adjunct to the clinical study of patients with ocular viral disease. Giemsa staining obscures nuclear details but remains an excellent technique for demonstrating cytoplasmic inclusions, giant cells, inflammatory cells, and microorganisms.