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July 1972

In Vitro Growth of Pure Cultures of Retinal Pigment Epithelium

Author Affiliations

New Haven, Conn; USA, Washington, DC; Bethesda, Md
From the Section of Ophthalmology, Yale University School of Medicine, New Haven, Conn (Dr. Albert), the Ophthalmic Pathology Branch, Armed Forces Institute of Pathology, Washington, DC (Dr. Tso), and the Pathologic Anatomy Branch, National Cancer Institute, National Institutes of Health, Bethesda, Md (Dr. Rabson).

Arch Ophthalmol. 1972;88(1):63-69. doi:10.1001/archopht.1972.01000030065014

Retinal pigment epithelium (RPE) was isolated from five human eyes and grown in vitro as an explant culture. Cultures of choroid and RPE with choroid were similarly established for comparison. The tissues were maintained in culture for periods up to six months, and the morphologic characteristics of the cells were studied and documented. Mosaic, tubular, and spindle growth patterns were observed to evolve from the RPE. Certain cytologic features suggestive of dedifferentiation and neoplasia were noted. There was a general decrease in the amount of pigment in the cells as tissue culture progressed. The appearance of the cells grown in tissue culture is compared with that of RPE seen in normal and pathologic situations.