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November 1972

Mechanisms of the Host Response in the Eye: VI. Immune and Toxic Stimulation of Inflammation in the Germ-Free Guinea Pig

Author Affiliations

San Francisco; Bethesda, Md
From the Eyetis Foundation, San Francisco (Dr. Aronson); and the Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md (Dr. McMaster).

Arch Ophthalmol. 1972;88(5):533-539. doi:10.1001/archopht.1972.01000030535014

Germ-free and conventional guinea pigs have been conjunctivally challenged with a topical immunogen, ie, albumin, and a topical nonnecrotizing toxic agent, ie, acacia extract. Following immunogenic challenge, germ-free guinea pigs demonstrated appreciably more severe anterior ocular inflammation than conventional animals, which correlated with higher humoral antibody titers. Similarly, germ-free animals demonstrated more severe inflammation when challenged with the toxic agent, acacia. In this circumstance, disease severity correlated with serum γ-globulin levels. These studies readily demonstrate the superiority of germ-free guinea pigs in acute toxicity testing.

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