A single drop of tritium-labeled 0.1% fluorometholone suspension was administered topically to intact eyes of anesthetized and unanesthetized rabbits. Aqueous humor levels of steroid were monitored as a function of time. The aqueous humor drug concentration-time profile for anesthetized rabbits is considerably different from that for unanesthetized animals. Anesthetized animals show a shift in the time to achieve a maximum aqueous humor steroid concentration, which is ascribed to a change in rate-limiting step from corneal absorption control to dissolution control. In addition, aqueous humor drug levels in the anesthetized rabbits were dependent on position of the animals and level of anesthesia.
Sieg JW, Robinson JR. Corneal Absorption of Fluorometholone in Rabbits: A Comparative Evaluation of Corneal Drug Transport Characteristics in Anesthetized and Unanesthetized Rabbits. Arch Ophthalmol. 1974;92(3):240–243. doi:10.1001/archopht.1974.01010010248016
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