Dogs inoculated intravenously with attenuated canine adenovirus type 1 developed anterior segment inflammation and corneal edema. During the stage of mild anterior uveitis, virus was isolated from the aqueous fluid, and by electron microscopy, viral replication was found to occur in corneal endothelial cells.
Later, at the stage of severe anterior uveitis with corneal edema, virus was not isolated from the aqueous fluid and cells containing intranuclear (replicating) virus were not found. At this stage, many inflammatory cells had infiltrated the anterior chamber and contained numerous membrane-bound viral aggregates (viralantibody complexes). Phagocytized viralantibody complexes were present in the areas of most prominent endothelial cell destruction. Peripheral to the principal lesion sites, inflammatory cells had dissected the endothelium from Descemet membrane. After recovery from the disease, an intact endothelial cell layer was present.
Gustavo Aguirre, Leland Carmichael, Stephen Bistner. Corneal Endothelium in Viral Induced Anterior UveitisUltrastructural Changes Following Canine Adenovirus Type 1 Infection. Arch Ophthalmol. 1975;93(3):219–224. doi:10.1001/archopht.1975.01010020227012