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September 1975

Prevention of Experimental Allergic Uveitis: Treatment with Methotrexate

Author Affiliations

From the Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md (Drs. McMaster and Owens); the Department of Ophthalmology, Georgetown University Medical School, Washington, DC (Dr. Wong); and the Department of Pathology, Washington University School of Medicine, St. Louis, Mo (Dr. Kyriakos).

Arch Ophthalmol. 1975;93(9):835-837. doi:10.1001/archopht.1975.01010020719010

These experiments were undertaken to determine if methotrexate therapy, initiated after immunization, could prevent the development of experimental allergic uveitis. Strain 13 guinea pigs were immunized with Strain 13 guinea pig retina-uvea extract that had been emulsified in Freund complete adjuvant. Some were treated with methotrexate twice a week until the 21st day. Each week, all of their eyes were examined with a slit-lamp. At the end of the study, some were skintested, and the sera of selected animals were tested by immunodiffusion for antibody. The eyes of certain groups were examined histologically. Results show methotrexate prevented the development of this type of uveitis, even when therapy was initiated seven days after immunization. The disease did not appear after therapy was stopped. Methotrexate also inhibited the development of skin sensitivity and antibody to retina-uvea antigen.

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