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November 1976

McCarey-Kaufman Technique of Corneal Storage Before Penetrating Keratoplasties in Rabbits: An Evaluation

Author Affiliations

From the Pocklington Eye Transplantation Research Unit, Department of Clinical Ophthalmology, Institute of Ophthalmology, London. Dr Breslin is now with the Department of Ophthalmology, University of Toronto.

Arch Ophthalmol. 1976;94(11):1976-1979. doi:10.1001/archopht.1976.03910040682016

• The endothelial function of rabbit corneas stored in modified tissue culture medium (McCarey-Kaufman medium) for periods of 4, 8, or 12 days was studied after penetrating keratoplasty using the specular microscope and the response to temperature reversal.

Our results indicate that rabbit donor corneas stored in McCarey-Kaufman (M-K) medium are suitable for penetrating keratoplasty after storage periods as long as 12 days.

The limitations of using graft clarity or the morphologic appearance of the endothelium as an index of the success of a penetrating keratoplasty in the rabbit are discussed.