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March 1977

Bacteriologic Safety of Using Tissue-Culture Storage-Reply

Arch Ophthalmol. 1977;95(3):519. doi:10.1001/archopht.1977.04450030161025

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In Reply.—Drs Mishler and Keates report their inability to culture bacteria from corneal-scleral remnants and McCarey-Kaufman media immediately following keratoplasty. Lacking the details of their technique, I cannot comment on it. These authors question the merit of delaying surgery for 48 hours while awaiting the result of a culture report. We suggest that the interval between enucleation and keratoplasty not be regarded as a delay, even though we used the word delay in our article. Comparing moist chamber to tissue-culture medium storage, advocates of the latter emphasize the improved viability of corneas kept for more than 48 hours in a tissue-culture medium. The advantage of tissueculture storage compared with moistchamber storage for periods of less than 48 hours is questionable.

The development of a tissue culture fluid as a means of extending the lifespan of a donor cornea deserves support and encouragement. However, since we presented our paper last

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