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June 1978

Corneal Storage at Room Temperature

Author Affiliations

From the Department of Ophthalmology, College of Medicine, University of Florida, Gainesville. Dr Sachs is now with the Soroca Medical Center, Beer-Sheva, Israel; Dr Goldman is with the Department of Ophthalmology, Montefiore Hospital, Bronx, NY; Dr Kaufman is with the Department of Ophthalmology, Louisianae State University School of Medicine, New Orleans.

Arch Ophthalmol. 1978;96(6):1075-1077. doi:10.1001/archopht.1978.03910050595022

• Short-term eye banking is based mainly on moist chamber and McCarey-Kaufman medium (M-K medium) preservation. BOth involve a controlled 4 C temperature for storage. Warming the cornea to room temperature, however, drastically affects the endothelial viability. On enzymatic staining and histological study, the M-K medium-stored rabbit corneas had more normal endothelium than did "moist chamber" eyes when storage was prolonged for seven days at room temperature. In human corneas that were kept at 4 C for 24 hours and then exposed to a temperature of 25 C, destruction of organelles had occurred by six hours and was increased by 12 hours. Corneas that were kept in M-K medium had relatively intact endothelium after four days, but cell disruption and vacuolation was present by the seventh day. The M-K medium, therefore, affords protection to tissue warmed to room temperature, where metabolic activity is resumed.

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