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July 1978

Efficacy of Penicillin G, Cefazolin, and Gentamicin in M-K Medium at 4° C

Author Affiliations

From the Departments of Ophthalmology (Dr Baum and Ms Kane) and Medicine, Infectious Disease Service (Dr Barza), New England Medical Center Hospital and Tufts University School of Medicine, Boston.

Arch Ophthalmol. 1978;96(7):1262-1264. doi:10.1001/archopht.1978.03910060088019

• Corneal-scleral shells are routinely stored in McCarey-Kaufman (M-K) medium at 4° C. Antibiotics are added to the medium to suppress bacterial growth. We examined the antibacterial activity of penicillin G potassium, 100 μg/ml, cefazolin sodium, 100 μg/ml, and gentamicin sulfate, 20 μg/ml against test organisms in M-K medium at 4°, 23°, and 37° C for periods up to 18 hours. Within the limits of the experimental conditions, none of the antibiotics used could be relied on to sterilize an inoculum of sensitive bacteria in M-K medium at 4° C, the recommended storage temperature.

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