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January 1982

Ocular Adnexal Lymphoid Tumors: Correlative Ultrastructural and Immunologic Marker Studies

Author Affiliations

From the Departments of Ophthalmology and Pathology, The New York Hospital-Cornell Medical Center, and the Manhattan Eye, Ear, and Throat Hospital, New York (Drs Jakobiec and Iwamoto); the Department of Pathology, Columbia University, College of Physicians and Surgeons, New York (Dr Knowles); and the Eye Bank for Sight Restoration, Inc, New York (Dr Jakobiec).

Arch Ophthalmol. 1982;100(1):84-98. doi:10.1001/archopht.1982.01030030086007

• Twenty-two ocular adnexal lymphoid infiltrates were analyzed by electron microscopy as well as immunologically and cytochemically. Five reactive polyclonal lesions were found to be preponderantly composed of small mature lymphocytes (presumably T cells) with clumped nuclear chromatin, sparse cytoplasmic organelles, and numerous monoribosomes. In 11 monoclonal B-cell lesions, both the 1-μm plastic sections examined by light microscopy and the electron micrographs disclosed immature cells, with more dispersed nuclear chromatin, prominent nucleoli, abundant cytoplasmic polyribosomes, and increased numbers of mitochondria and strands of endoplasmic reticulum (particularly in plasmacytoid lesions). The remaining six monoclonal B-cell lesions were composed of comparatively well-differentiated cells requiring electron microscopy to show somewhat more prominent nucleoli, slightly less dense clumping of the nuclear chromatin, increased numbers of mitochondria and short segments of rough-surfaced endoplasmic reticulum, and monoribosomes rather than polyribosomes. The importance of distinguishing this group of well-differentiated monoclonal lesions from the less well-differentiated ones was underscored by the results of the follow-up examinations, in that no evidence of extraorbital disease has been discovered in the former group, while a 50% incidence occurred in the latter.

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