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May 1982

Centrifugal Cytology of Ocular Fluids

Author Affiliations

From the Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine (Drs Stulting and Clarkson); and the Papanicolaou Cancer Research Institute (Dr Leif and Ms Bobbitt), Miami. Dr Stulting is now with Emory University Clinic, Atlanta. Dr Leif is now with Coulter Electronics, Hialeah, Fla.

Arch Ophthalmol. 1982;100(5):822-825. doi:10.1001/archopht.1982.01030030826021

• Centrifugal cytology is a new technique for the preparation of ocular fluids for cytologic examination. It differs from conventional cytocentrifuge techniques, since fixation is carried out simultaneously with centrifugation. It avoids air-drying artifacts and can be applied to small (50 μL) or large (200 mL or more) sample volumes and dilute cell suspensions, such as might be obtained by anterior chamber paracentesis, vitreous aspiration, or vitrectomy. The resultant preparation is a permanent, stained, well-preserved, flattened, homogeneous cell dispersion suitable for detailed cytologic evaluation.