• Twenty biopsy specimens of the human conjunctival epithelial surface with its associated mucus from normal subjects and contact lens wearers with biomicroscopic evidence of excess mucus were studied by scanning electron microscopy. Mucus existed in strands, sheets, and granules; each morphologic type of mucus was observed in all samples. No qualitative difference in morphologic features of mucus was found between normal subjects and those with excess mucus. Mucous forms observed by scanning electron microscopy closely resembled structures previously shown by biomicroscopic and light microscopic techniques, providing evidence that careful scanning electron microscopic preparation of biopsy specimens containing mucus may not greatly alter in situ morphologic characteristics of mucus.
Greiner JV, Korb DR, Covington HI, Peace DG, Allansmith MR. Human Ocular MucusScanning Electron Microscopic Study. Arch Ophthalmol. 1982;100(10):1614–1617. doi:10.1001/archopht.1982.01030040592007
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