• The ultrastructure of Descemet's membrane was studied in 24 eyes, at least two eyes from patients in each of the first nine decades of life. Both the anterior banded layer and the posterior nonbanded layer were regular, predictable structures, with no extra layers or significant substructures present. The anterior banded layer was present in the eye of a fetus of five months' gestation. The thickness of the anterior layer was approximately 3 μm and similar in specimens from patients of all ages. The thickness of the posterior nonbanded layer of Descemet's membrane increased significantly with age, averaging approximately 2 μm at age 10 years and 10 μm at age 80 years.
Johnson DH, Bourne WM, Campbell RJ. The Ultrastructure of Descemet's Membrane: I. Changes With Age in Normal Corneas. Arch Ophthalmol. 1982;100(12):1942–1947. doi:10.1001/archopht.1982.01030040922011
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