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To the Editor.
—The article by Drs Gibralter and Jakobiec entitled "Supravital and Vital Staining of Diseased Corneal Endothelium in Whole-Mount Preparations," in the August Archives (1982;100:1308-1312), described a new staining technique (a combination of trypan blue and alizarin red S) for quantitative evaluation of corneal endothelial cells. The method was first described by us in Investigative Ophthalmology (1976;15:1000-1002).
Spence D, Peyman GA. Diseased Corneal Endothelium. Arch Ophthalmol. 1983;101(3):488. doi:10.1001/archopht.1983.01040010488044
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