Diseased Corneal Endothelium

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To the Editor.  —The article by Drs Gibralter and Jakobiec entitled "Supravital and Vital Staining of Diseased Corneal Endothelium in Whole-Mount Preparations," in the August Archives (1982;100:1308-1312), described a new staining technique (a combination of trypan blue and alizarin red S) for quantitative evaluation of corneal endothelial cells. The method was first described by us in Investigative Ophthalmology (1976;15:1000-1002).