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August 1984

Experimental Murine Cytomegalovirus Infection of Ocular Structures

Author Affiliations

From the Division of Pediatric Neurology, the Department of Pediatrics and Neurology, the University of Iowa College of Medicine, Iowa City (Dr Bale, Ms O'Neil); the Laboratory of Molecular Genetics, National Institutes of Neurologic Communicative Disorders and Stroke, the National Institute of Health, Bethseda, Md (Dr Hogan); and the Division of Pediatric Infectious Disease, Department of Pediatrics, University of Utah School of Medicine, Salt Lake City (Dr Kern).

Arch Ophthalmol. 1984;102(8):1214-1219. doi:10.1001/archopht.1984.01040030984032

• To study experimental cytomegalovirus (CMV) infection of ocular structures, 3-week-old Swiss-Webster mice were inoculated intraperitoneally with a 0% to 20% lethal inoculum of murine CMV (MCMV). Murine cytomegalovirus was recovered from homogenates of eye tissues on days 3, 5, and 7 after inoculation. Peak virus titers, mean of 2.93 ± 0.67 log plaque-forming units of MCMV per gram of ocular tissue occurred on day 5. Urine cytomegalovirus was recovered from explant cultures of eye and optic nerve 14, 21, 60, 90, and 120 days after MCMV inoculation. Murine cytomegalovirus also persisted in intraocular fluids for as long as 90 days. Murine cytomegalovirus infection was confirmed by immunofluorescence antibody staining and transmission electron microscopy. These experiments indicate that MCMV infects the ocular tissues of mice during systemic MCMV infection, and persists in ocular tissues for as long as 120 days after infection. These studies may be directly relevant to ocular disorders that occur during acquired CMV infections of humans, and suggest that ocular tissues may be a site of CMV persistence or latency.

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