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June 1985

Retinal S Antigen and Retinoblastoma: A Monoclonal Antibody Histopathologic Study

Author Affiliations

From the Department of Clinical Research and the Retina Service (Dr Donoso) and the Department of Pathology (Ms Arbizo), Wills Eye Hospital, Philadelphia, and the Department of Ophthalmology, University of Iowa Hospital and Clinics, Iowa City (Dr Folberg).

Arch Ophthalmol. 1985;103(6):855-857. doi:10.1001/archopht.1985.01050060115039

• Using monoclonal antibody MAbA9-C6, which defines an epitope of retinal S antigen retained in fixed-tissue sections, we investigated S-antigen immunoreactivity in human fetal eyes from different gestational periods and 19 cases of retinoblastoma. S-antigen immunoreactivity was first observed in the outermost portion of the neuroblastic cell layer in the retina of a human fetus at 18 weeks' gestation, a stage preceding photoreceptor differentiation, and at all later stages examined thereafter. All adult eyes containing normal photoreceptors bound MAbA9-C6. In retinoblastomas, several different patterns of S-antigen immunoreactivity were recognized. In seven cases, S-antigen immunoreactivity appeared to be associated with normal photoreceptor elements incorporated into the growing tumor. S-antigen immunoreactivity was associated with fleurettes within the retinoblastoma in three cases and Flexner-Wintersteiner rosettes in seven cases. In 11 cases, S-antigen immunoreactivity was associated with isolated tumor cells. Our results show that S antigen may be a useful marker in the study of the embryologic development of the human retina and antigenic expression and modulation in retinoblastomas.

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