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September 1985

Identification of Proteins in Contact Lens Surface Deposits by Immunofluorescence Microscopy

Arch Ophthalmol. 1985;103(9):1269-1270. doi:10.1001/archopht.1985.01050090019003

To the Editor.  —A recent report in the Archives by Gudmundsson and associates,1 my colleagues at the Eye Research Institute of Retina Foundation, Boston, quoted some of my work, but I was not aware of the article before its publication. I agree that the authors have demonstrated that several tear proteins are adsorbed on worn soft (hydrogel) contact lenses and that these proteins are sufficiently undenatured so as to be detected by immunofluorescence microscopy. However, I disagree with their interpretation of the results. First, the fact that there are some undenatured proteins on the lenses does not preclude the presence of denatured proteins.2 The immunologic technique does not detect denatured proteins. In addition, Mannucci et al3 reported at the recent Sixth International Congress of Eye Research in Alicante, Spain, that circular dichroism showed denatured lysozyme in worn hydrogel lenses.Second, the fact that the immunofluorescence technique detected