[Skip to Navigation]
September 1985

Identification of Proteins in Contact Lens Surface Deposits by Immunofluorescence Microscopy-Reply

Arch Ophthalmol. 1985;103(9):1270. doi:10.1001/archopht.1985.01050090019004

This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.


In Reply.  —Dr Refojo points out that presence of undenatured proteins on the lenses does not preclude the presence of denatured proteins. We agree with him and would like to stress that we did not make statements to the contrary in our aforereferenced article, "Identification of Proteins in Contact Lens Surface Deposits by Immunofluorescence Microscopy."Dr Refojo's second comment was regarding the staining of proteins in the material or matrix of the lens. We would like to explain that the lenses are cut transversely. The cut side (not only the outer surface) is also treated with the antisera. Therefore, there would be a good possibility of detectable staining if there were any undenatured proteins inside the material.As stated in our article, the relationship of cleaning and disinfection of the lenses to deposits was outside the scope of the study. However, we agree that processes of disinfection, especially heat disinfection,

First Page Preview View Large
First page PDF preview
First page PDF preview
Add or change institution