—We wish to thank Dr Thaller for his thoughtful reading of our article, his comments, and this opportunity to present further clarification of our study.The "estimate of the expected error" is calculated from a combination of the gas laws of Boyles and Charles, ie, P1 × V1/T1 = P2 × V2/T2, where P is the absolute pressure, V is the volume, and T is the temperature in degrees kelvin. Calculation is then 760 mm Hg × 0.5 mL/293 °K = 775 mm Hg × V2/309 °K, where P1 is 760 mm Hg atmospheric pressure; V, is 0.5 mL injected gas; T, is 20 °C + 273 °C, where room temperature is 20 °C; P2 is intraocular pressure of 15 mm Hg plus atmospheric pressure; V2 is new gas volume; and T2 is anterior chamber temperature of
Wolf GL, Hartung J. Effect of Nitrous Oxide on Gas Bubble Volume-Reply. Arch Ophthalmol. 1985;103(9):1272–1274. doi:10.1001/archopht.1985.01050090022008
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