Molecular defects that contribute to human hereditary diseases are being revealed at an accelerated rate by the use of recombinant DNA technology. Cloned genes and molecular genetic markers have created a new phase in the study of inherited eye disorders. This editorial summarizes some of the principles of the new approach and comments on its current application and potential impact on the field of ophthalmology.
See also p 1055.
Fragments of DNA from human chromosomes have been cloned, that is, exact copies of the original molecules have been produced in large quantities. Cloning enables scientists to isolate human genes from a collection of human-bacteriophage recombinant DNA molecules that are propagated in a bacterial host. The collection, known as a genomic library, may contain a million bacteriophage clones, and each clone carries a different fragment of human DNA that may be part of a gene. The desired DNA fragment is identified
Wong F. A New Phase in the Study of Human Inherited Eye Diseases. Arch Ophthalmol. 1987;105(8):1039–1041. doi:10.1001/archopht.1987.01060080041024
Coronavirus Resource Center
Customize your JAMA Network experience by selecting one or more topics from the list below.
Create a personal account or sign in to: