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November 1987

Dry Vitreous Biopsy With Gas-Vitreous Exchange

Arch Ophthalmol. 1987;105(11):1478. doi:10.1001/archopht.1987.01060110024009

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To the Editor.  —Vitreous biopsy techniques have become important in establishing the diagnosis of intraocular lymphoma and in finding the causative agents in acute retinal necrosis. If one wishes to perform serologic testing for viral titers on vitreous biopsy specimens, it is important to avoid dilution of the specimens. Thus, to obtain a dry vitreous biopsy specimen, one is limited to the amount that can be aspirated before the eyeball collapses too much. We report a technique that has permitted the removal of over 3 mL of vitreous from an eye with acute retinal necrosis, while preventing collapse of the globe and without dilution of the vitreous specimen.Two sclerotomy sites are made, as in a standard vitrectomy. A vitreous cutter is inserted in the first sclerotomy, with the tip placed in the midvitreous. The cutting mechanism is attached to the console, while the suction line is attached to a

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