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February 1988

In Vitro Melanoma Cell Growth After Preenucleation Radiation Therapy

Author Affiliations

From the Departments of Ophthalmology (Drs Kenneally, Farber, and Smith) and Radiotherapy (Dr Devineni), Washington University School of Medicine, St Louis.

Arch Ophthalmol. 1988;106(2):223-224. doi:10.1001/archopht.1988.01060130233034

• The in vitro efficacy of 20 Gy (2000 rad) of external beam irradiation delivered to patients with choroidal melanomas prior to enucleation was investigated in 11 patients whose tumors were grown in cell culture. Phase-contrast microscopy was used to compare growth patterns between irradiated and nonirradiated tumors. Cell types were determined by histologic stains, and electron microscopy identified intracytoplasmic melanin. Irradiated melanomas did not grow and did not attach to culture flasks, thus demonstrating that preenucleation irradiation alters the in vitro growth of melanoma cells.

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