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September 1989

DNA Content Analysis of Uveal Melanoma

Author Affiliations

San Francisco, Calif
Washington, DC
Louisville, Ky

Arch Ophthalmol. 1989;107(9):1278. doi:10.1001/archopht.1989.01070020348006

To the Editor.  —DNA content flow cytometric analysis and morphometric determination of SD of necleolar area (SDNA) correlate with uveal melanoma mortality.1,2 In this retrospective study we assessed the relative prognostic accuracy of these techniques.One hundred twenty-seven enucleated uveal melanomas submitted to the Armed Forces Institute of Pathology, Washington, DC, prior to 1973 were analyzed in a masked manner. The DNA content was studied on deparaffinized 50-μm stained sections (Chromomycin A-3) using a cytometer (FACS IV, Becton-Dickinson, Mountain View, Calif).2 Histogram peaks with coefficients of variation less than 8.0 were accepted. Single-peak histograms were assumed to be diploid. The lowest DNA content spike in a multiple peak was considered diploid and the others aneuploid. Degree of aneuploidy was expressed as a "DNA index" that equaled the ratio of the aneuploid to diploid peaks; diploid tumors were assigned a DNA index of 1.0.Nucleolar areas of 200 randomly