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October 1989

Freezing and Acanthamoeba

Author Affiliations

Cleveland, Ohio

Arch Ophthalmol. 1989;107(10):1420. doi:10.1001/archopht.1989.01070020494008

To the Editor.  —We read with interest the article by Matoba and associates1 in the March 1989 issue of the Archives and compliment the authors on a well-executed study. We wish, however, to clarify the authors' interpretation of results of a similar experiment we conducted using cryotherapy alone to eliminate Acanthamoeba organisms.2 Although Matoba and associates reported that their results were in "distinct contrast" to ours, in essence, it appears that they have demonstrated results that are consistent, ie, Acanthamoeba polyphaga and Acanthamoeba castellanii cysts can survive a freeze-thaw-refreeze trial. In fact, we made no attempt to quantitate the organisms surviving the cryotherapy in our experiment and therefore do not know if our cryotherapeutic trials had "no effect" on the viability of Acanthamoeba cysts as Matoba and associates have stated. If our statement that "all Acanthamoeba cysts survived" was interpreted as a quantitative assessment, we apologize for the

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