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October 1989

Freezing and Acanthamoeba-Reply

Author Affiliations

Houston, Tex

Arch Ophthalmol. 1989;107(10):1420. doi:10.1001/archopht.1989.01070020494009

In Reply.  —We value the comments of Drs Meisler and Rutherford and appreciate their interest in our article. In fact, it was our impression that their experiments had demonstrated little or no effect of cryotherapy on Acanthamoeba cysts based on the following statements: "Motile trophozoites were observed by day 1 in all trials testing A polyphaga cysts,"1 "Motile trophozoites were observed by day 3 in all trials testing A castellanii cysts,"1 and "In this study, all Acanthamoeba cysts survived cryotherapeutic methods."1 Therefore, we appreciate their comments illuminating the fact that no attempts were made to quantify their results. However, we believe that our results are, in some respects, in contrast to their data because A castellanii cysts appeared to be eradicated in two of three experiments in which we could not detect any evidence of viable trophozoites by day 12 following cryotherapy.2 Thus, our data raise

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