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January 1990

Pathogenicity of Acanthamoeba and a Corynebacterium in the Rat Cornea

Author Affiliations

From the Departments of Ophthalmology (Mr Badenoch and Dr Coster) and Clinical Microbiology (Dr Johnson), Flinders Medical Centre, Bedford Park, South Australia; and the State Water Laboratory (Mr Christy), Bolivar, South Australia.

Arch Ophthalmol. 1990;108(1):107-112. doi:10.1001/archopht.1990.01070030113040

Acanthamoeba keratitis is a sight-threatening disease that is difficult to treat. The development of an animal model is necessary for many of the studies required to improve visual outcome in human patients. A rat model is proposed that is dependent on coinoculation of amebae and corynebacteria into the corneal stroma. The infective dose was determined for a virulent Acanthamoeba isolate and was used to screen 17 other isolates, including 7 from the human cornea. A total of 6 were infective in the rat cornea. The model should be useful for controlled in vivo studies of this intractable condition.