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May 1990

Immunocytochemical Localization of Gentamicin in the Rabbit Retina Following Intravitreal Injection

Author Affiliations

From the Howe Laboratory of Ophthalmology (Drs Tabatabay, Young, and D'Amico), the Retina Service (Drs Young and D'Amico), and the Cornea Service (Dr Kenyon), the Massachusetts Eye and Ear Infirmary, the Department of Ophthalmology, Harvard Medical School, Boston, Mass.

Arch Ophthalmol. 1990;108(5):723-726. doi:10.1001/archopht.1990.01070070109046

• The localization of gentamicin in the retina after a single intravitreal injection in the rabbit eye was examined by indirect immunofluorescent staining with goat antigentamicin antiserum. Eight hours after the injection of 400 μg of gentamicin, staining was observed in the ganglion cell layer, the inner plexiform layer, the inner nuclear layer, and the photoreceptors. By 12 hours, the staining was also observed in the retinal pigment epithelium. However, by 24 hours the staining was predominantly found in the retinal pigment epithelium and choriocapillaris, and only occasional staining was seen scattered in the neurosensory retina. At 36 to 48 hours, the labeling was confined to the retinal pigment epithelium and choriocapillaris. Electron microscopy confirmed the cytoplasmic localization of gentamicin in the retina.

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