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April 1991

Punch Biopsy Technique for the Ophthalmologist

Author Affiliations

Hershey, Pa

Arch Ophthalmol. 1991;109(4):464-465. doi:10.1001/archopht.1991.01080040024007

To the Editor.  —I was pleased to read the report by Warren and associates1 describing their experience with the disposable skin biopsy punch. In 1981, we described the use of an essentially identical instrument, the Baker's disposable biopsy punch, for obtaining tarsal conjunctival biopsy specimens.2Since our initial report, we have modified our procedure. The biopsy "button" is outlined using the Acuderm punch described by Warren and associates. (We have discontinued use of the Baker's punch due to its unavailability.) As the biopsy specimen is outlined, the punch penetrates the tissue to the desired depth. A suture is passed through the base of the specimen and used to provide traction as the biopsy button is amputated at its base with fine scissors or a scalpel.After the needle is safely disposed of, the specimen is permitted to remain on the suture during fixation to facilitate specimen identification, orientation,

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