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October 1992

A Randomized, Prospective, Double-Masked Clinical Trial of Optisol vs DexSol Corneal Storage Media

Author Affiliations

From the Department of Ophthalmology, Case Western Reserve University and University Hospitals of Cleveland, Ohio (Drs Lass [Image Analysis Reading Center], Reinhart, Bruner, and Cano and Ms Patel); the Department of Ophthalmology, Mayo Clinic and Mayo Foundation, Rochester, Minn (Drs Bourne and Maguire); the Departments of Ophthalmology (Drs Musch [Data Coordinating Center], Sugar, Meyer, and Soong) and Epidemiology (Dr Musch), University of Michigan, Ann Arbor; Chiron Ophthalmics Inc, Irvine, Calif (Dr Gordon); and the Department of Ophthalmology, Boston (Mass) University (Dr Laing). Chiron Ophthalmics Inc had the following proprietary relationships with these authors: Dr Lass received support for image analyses performed, Drs Musch and Laing are consultants, and Dr Gordon is an employee. The other authors have no proprietary interest in this work.

Arch Ophthalmol. 1992;110(10):1404-1408. doi:10.1001/archopht.1992.01080220066022

• We compared Optisol and DexSol, two chondroitin-sulfate-based media for corneal storage at 4°C, by transplanting 31 donor cornea pairs (one cornea stored in Optisol and its mate in DexSol for 20 to 134 hours) into 31 pairs of recipients (62 patients). All grafts were clear 1 year after transplantation except for one primary donor failure (Optisol group). Optisol-stored corneas were significantly thinner than DexSol-stored corneas after cardinal suture placement (0.64 mm vs 0.76 mm) and at the end of surgery (0.69 mm vs 0.78 mm); at all points afterward through 1 year the two groups did not differ. The activity of two lysosomal enzymes released into the media during storage, α-mannosidase and α-glucosidase, was lower in the Optisol group. Epithelial status and all endothelial morphometric parameters, except the figure coefficient at 1 year, did not differ between the two groups before surgery and 3, 6, and 12 months after surgery. Mean endothelial cell loss at 1 year was 15% for the Optisol group and 21% for the DexSol group (P=.22). Thus, Optisolstored corneas were thinner during surgery than DexSol-stored corneas and there was less lysosomal enzyme activity in the Optisol medium after tissue storage. There were no significant differences in postoperative clinical or endothelial morphometric parameters, however.

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